Intensivkurs Proteomics
Proteomics Practical Course
Lehrveranstaltung 240790810 im WS 2022/3
Basisdaten
LV-Art | Übung |
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Umfang | 3 SWS |
betreuende Organisation | Lehrstuhl für Proteomik und Bioanalytik (Prof. Küster) |
Dozent(inn)en |
Bernhard Küster Christina Ludwig Annika Schneider Matthew The |
Termine |
Mo, 09:00–17:00, EG L 12 Mo, 09:00–17:00, DG L 01 sowie 10 Termine in Gruppen |
Zuordnung zu Modulen
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WZ2439: Proteomics: Analytische Grundlagen und Biomedizinische Anwendungen / Proteomics: Analytical Basics and Biomedical Applications
Dieses Modul ist in den folgenden Katalogen enthalten:- Fokussierungsrichtung Biosensorik im M.Sc. Biomedical Engineering and Medical Physics
weitere Informationen
Lehrveranstaltungen sind neben Prüfungen Bausteine von Modulen. Beachten Sie daher, dass Sie Informationen zu den Lehrinhalten und insbesondere zu Prüfungs- und Studienleistungen in der Regel nur auf Modulebene erhalten können (siehe Abschnitt "Zuordnung zu Modulen" oben).
ergänzende Hinweise | In this exercise course, the participants will learn mass spectrometry-based methods and evaluation processes that allow identification as well as quantification of proteins. In every module the participants will work with data from a case study, which aims at identifying specific protein interaction partners of the clinical kinase inhibitor Lestaurtinib. Along with this case study, the participants will get familiar with the three steps required for every proteomic experiment: i) sample preparation, ii) mass spectrometric measurement, iii) (statistical) data analysis. The focus of the course will be on statistical data analysis. Here the participants will learn how to first manually annotate fragment ion spectra and then use “Sequence-Tags” for spectra interpretation. Further, they will learn how information about amino acid sequences and masses can be used for database searches. Finally, they will analyze data from an automated database search. Here the best matches between peptide sequences and acquired MS spectra were automatically identified and the underlying error rates were estimated. The used algorithms will be explained in detail. In the last part of the course the participants will learn that proteomics data can also be used for protein quantification. In this context, different visualization tools for quantitative data will be tested (principle component analysis, hierarchical clustering analysis, etc.) and a statistical evaluation of protein concentration differences between samples will be carried out (T-test analysis and volcano plots). All content stated above will be taught in a computer room and participants will perform exercises and data analyses themselves using software tools provided. There will be an intensive interaction between teachers and course participants. |
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Links | TUMonline-Eintrag |
Gleiche Lehrveranstaltungen (z. B. in anderen Semestern)
Semester | Titel | Dozent(en) | Termine |
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WS 2023/4 | Proteomics Practical Course | Abele, M. Küster, B. Ludwig, C. Schneider, A. The, M. |
Mo, 09:00–17:00, EG L 12 Mo, 09:00–17:00, DG L 01 sowie Termine in Gruppen |
SS 2023 | Proteomics Practical Course | Küster, B. Ludwig, C. Schneider, A. The, M. Wilhelm, S. |
Mo, 09:00–17:00, DG L 01 Di, 09:00–17:00, DG L 01 Mo, 09:00–16:00, DG L 01 Mo, 09:00–16:00, EG L 12 |
SS 2022 | Intensivkurs Proteomics | Küster, B. Ludwig, C. Meng, C. Wilhelm, S. |
Mo, 09:00–16:00, DG L 01 Mo, 09:00–16:00, EG L 12 sowie Termine in Gruppen |
WS 2021/2 | Intensivkurs Proteomics | Küster, B. Ludwig, C. Meng, C. Wilhelm, S. |
Mo, 09:00–17:00, EG L 12 Mo, 09:00–17:00, DG L 01 sowie Termine in Gruppen |
SS 2021 | Proteomics Practical Course | Küster, B. Ludwig, C. Meng, C. Wilhelm, S. |
Mo, 09:00–16:00, EG L 12 Di, 09:00–16:00, DG L 01 sowie einzelne oder verschobene Termine sowie Termine in Gruppen |
WS 2020/1 | Intensivkurs Proteomics | Küster, B. Ludwig, C. Wilhelm, M. Wilhelm, S. |
Mo, 09:00–17:00, DG L 01 Mo, 09:00–17:00, EG L 12 sowie Termine in Gruppen |
SS 2020 | Proteomics Practical Course |
Ludwig, C.
Medard, G.
Meng, C.
Wilhelm, M.
Wilhelm, S.
Leitung/Koordination: Küster, B. |
Mo, 09:00–16:00, EG L 12 Mo, 09:00–16:00, EG L 12 Do, 09:00–16:00, EG L 12 |
SS 2020 | Proteomics Practical Course |
Ludwig, C.
Medard, G.
Meng, C.
Wilhelm, M.
Wilhelm, S.
Leitung/Koordination: Küster, B. |
Do, 09:00–17:00, EG L 12 Fr, 09:00–17:00, EG L 12 |
WS 2019/20 | Intensivkurs Proteomics |
Ludwig, C.
Meng, C.
Wilhelm, M.
Wilhelm, S.
Leitung/Koordination: Küster, B. |
Mo, 09:00–16:00, EG L 12 |
SS 2019 | Proteomics Practical Course |
Ludwig, C.
Medard, G.
Meng, C.
Wilhelm, M.
Wilhelm, S.
Leitung/Koordination: Küster, B. |
Mo, 09:00–16:00, EG L 12 |
WS 2018/9 | Intensivkurs Proteomics |
Ludwig, C.
Medard, G.
Meng, C.
Wilhelm, M.
Wilhelm, S.
Leitung/Koordination: Küster, B. |
Termine in Gruppen |
SS 2018 | Proteomics Practical Course | ||
WS 2017/8 | Intensivkurs Proteomics | ||
SS 2017 | Proteomics Practical Course | ||
WS 2016/7 | Intensivkurs Proteomics | ||
SS 2016 | Proteomics Practical Course | ||
WS 2015/6 | Intensivkurs Proteomics | ||
SS 2015 | Proteomics Practical Course | ||
WS 2014/5 | Intensivkurs Proteomics |