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Proteomics Practical Course

Course 0940790810 in SS 2021

General Data

Course Type exercise
Semester Weekly Hours 3 SWS
Organisational Unit Chair of Bioanalytics (Prof. Küster)
Lecturers Bernhard Küster
Christina Ludwig
Chen Meng
Stephanie Wilhelm
Dates Mon, 09:00–16:00, EG L 12
Tue, 09:00–16:00, DG L 01
and 1 singular or moved dates
and 10 dates in groups

Assignment to Modules

Further Information

Courses are together with exams the building blocks for modules. Please keep in mind that information on the contents, learning outcomes and, especially examination conditions are given on the module level only – see section "Assignment to Modules" above.

additional remarks In this exercise course, the participants will learn mass spectrometry-based methods and evaluation processes that allow identification as well as quantification of proteins. In every module the participants will work with data from a case study, which aims at identifying specific protein interaction partners of the clinical kinase inhibitor Lestaurtinib. Along with this case study, the participants will get familiar with the three steps required for every proteomic experiment: i) sample preparation, ii) mass spectrometric measurement, iii) (statistical) data analysis. The focus of the course will be on statistical data analysis. Here the participants will learn how to first manually annotate fragment ion spectra and then use “Sequence-Tags” for spectra interpretation. Further, they will learn how information about amino acid sequences and masses can be used for database searches. Finally, they will analyze data from an automated database search. Here the best matches between peptide sequences and acquired MS spectra were automatically identified and the underlying error rates were estimated. The used algorithms will be explained in detail. In the last part of the course the participants will learn that proteomics data can also be used for protein quantification. In this context, different visualization tools for quantitative data will be tested (principle component analysis, hierarchical clustering analysis, etc.) and a statistical evaluation of protein concentration differences between samples will be carried out (T-test analysis and volcano plots). All content stated above will be taught in a computer room and participants will perform exercises and data analyses themselves using software tools provided. There will be an intensive interaction between teachers and course participants.
Links TUMonline entry

Equivalent Courses (e. g. in other semesters)

SemesterTitleLecturersDates
WS 2022/3 Proteomics Practical Course Küster, B. Ludwig, C. Schneider, A. The, M. Mon, 09:00–17:00, DG L 01
Mon, 09:00–17:00, EG L 12
and dates in groups
SS 2022 Proteomics Practical Course Küster, B. Ludwig, C. Meng, C. Wilhelm, S. Mon, 09:00–16:00, EG L 12
Mon, 09:00–16:00, DG L 01
and dates in groups
WS 2021/2 Proteomics Practical Course Küster, B. Ludwig, C. Meng, C. Wilhelm, S. Mon, 09:00–17:00, DG L 01
Mon, 09:00–17:00, EG L 12
and dates in groups
WS 2020/1 Proteomics Practical Course Küster, B. Ludwig, C. Wilhelm, M. Wilhelm, S. Mon, 09:00–17:00, EG L 12
Mon, 09:00–17:00, DG L 01
and dates in groups
SS 2020 Proteomics Practical Course Ludwig, C. Medard, G. Meng, C. Wilhelm, M. Wilhelm, S.
Responsible/Coordination: Küster, B.
Thu, 09:00–17:00, EG L 12
Fri, 09:00–17:00, EG L 12
SS 2020 Proteomics Practical Course Ludwig, C. Medard, G. Meng, C. Wilhelm, M. Wilhelm, S.
Responsible/Coordination: Küster, B.
Mon, 09:00–16:00, EG L 12
Mon, 09:00–16:00, EG L 12
Thu, 09:00–16:00, EG L 12
WS 2019/20 Proteomics Practical Course Ludwig, C. Meng, C. Wilhelm, M. Wilhelm, S.
Responsible/Coordination: Küster, B.
Mon, 09:00–16:00, EG L 12
SS 2019 Proteomics Practical Course Ludwig, C. Medard, G. Meng, C. Wilhelm, M. Wilhelm, S.
Responsible/Coordination: Küster, B.
Mon, 09:00–16:00, EG L 12
WS 2018/9 Proteomics Practical Course Ludwig, C. Medard, G. Meng, C. Wilhelm, M. Wilhelm, S.
Responsible/Coordination: Küster, B.
dates in groups
SS 2018 Proteomics Practical Course
WS 2017/8 Proteomics Practical Course
SS 2017 Proteomics Practical Course
WS 2016/7 Proteomics Practical Course
SS 2016 Proteomics Practical Course
WS 2015/6 Proteomics Practical Course
SS 2015 Proteomics Practical Course
WS 2014/5 Proteomics Practical Course
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