Proteomics Practical Course
Intensivkurs Proteomics
Course 240790810 in WS 2018/9
General Data
| Course Type | exercise |
|---|---|
| Semester Weekly Hours | 3 SWS |
| Organisational Unit | Chair of Bioanalytics (Prof. Küster) |
| Lecturers |
Christina Ludwig Guillaume Medard Chen Meng Mathias Wilhelm Stephanie Wilhelm Responsible/Coordination: Bernhard Küster |
| Dates |
10 dates in groups |
Assignment to Modules
-
WZ2439: Proteomics: Analytische Grundlagen und Biomedizinische Anwendungen / Proteomics: Analytical Basics and Biomedical Applications
This module is included in the following catalogs:- Focus Area Bio-Sensors in M.Sc. Biomedical Engineering and Medical Physics
Further Information
Courses are together with exams the building blocks for modules. Please keep in mind that information on the contents, learning outcomes and, especially examination conditions are given on the module level only – see section "Assignment to Modules" above.
| additional remarks | Course participants learn methods and data analysis approaches for the identification of peptides and proteins by mass spectrometry. In order to effect this, participants receive samples of unknown proteins (or mixtures thereof) which are first separated by SDS-PAGE. Gel areas are excised from the gel in in-gel digested with the protease trypsin to generate peptides. Following deslating, the generated peptide mixtures are either analysed by MALDI TOF-TOF or by LC-coupled ESI-MS/MS. The obtained spectra are analysed in a number of ways. 1. Peptide mass fingerprinting:here, only intact peptide mass measurements are used to query protein sequence databases 2. Peptide sequence tag: in this method manual spectrum interpretation is used to derive a partial amino acid sequence from a tandem mass spectrum. The combination of AA sequence and fragment masses can be used to query a database to identify the underlying peptide. 3. automatic searches: here no manual spectrum analysis is performed. Instead, a computer algorithm is employed to match tandem mass spectra to peptide sequences. In this method, it is important to develp confidence criteria that allow an objective assessment of the plausibility of a spectrum to peptide match.. For all of the above, course participants are engaged in detailed interactions with their tutors. |
|---|---|
| Links | TUMonline entry |
Equivalent Courses (e. g. in other semesters)
| Semester | Title | Lecturers | Dates |
|---|---|---|---|
| SS 2024 | Proteomics Practical Course | Abele, M. Küster, B. Ludwig, C. Schneider, A. The, M. |
Mon, 09:00–16:00, DG L 01 Mon, 09:00–16:00, EG L 12 and dates in groups |
| WS 2023/4 | Proteomics Practical Course | Abele, M. Küster, B. Ludwig, C. Schneider, A. The, M. |
Mon, 09:00–17:00, DG L 01 Mon, 09:00–17:00, EG L 12 and dates in groups |
| SS 2023 | Proteomics Practical Course | Küster, B. Ludwig, C. Schneider, A. The, M. Wilhelm, S. |
Mon, 09:00–17:00, DG L 01 Tue, 09:00–17:00, DG L 01 Mon, 09:00–16:00, EG L 12 Mon, 09:00–16:00, DG L 01 |
| WS 2022/3 | Proteomics Practical Course | Küster, B. Ludwig, C. Schneider, A. The, M. |
Mon, 09:00–17:00, DG L 01 Mon, 09:00–17:00, EG L 12 and dates in groups |
| SS 2022 | Proteomics Practical Course | Küster, B. Ludwig, C. Meng, C. Wilhelm, S. |
Mon, 09:00–16:00, EG L 12 Mon, 09:00–16:00, DG L 01 and dates in groups |
| WS 2021/2 | Proteomics Practical Course | Küster, B. Ludwig, C. Meng, C. Wilhelm, S. |
Mon, 09:00–17:00, EG L 12 Mon, 09:00–17:00, DG L 01 and dates in groups |
| SS 2021 | Proteomics Practical Course | Küster, B. Ludwig, C. Meng, C. Wilhelm, S. |
Mon, 09:00–16:00, EG L 12 Tue, 09:00–16:00, DG L 01 and singular or moved dates and dates in groups |
| WS 2020/1 | Proteomics Practical Course | Küster, B. Ludwig, C. Wilhelm, M. Wilhelm, S. |
Mon, 09:00–17:00, EG L 12 Mon, 09:00–17:00, DG L 01 and dates in groups |
| SS 2020 | Proteomics Practical Course |
Ludwig, C.
Medard, G.
Meng, C.
Wilhelm, M.
Wilhelm, S.
Responsible/Coordination: Küster, B. |
Mon, 09:00–16:00, EG L 12 Mon, 09:00–16:00, EG L 12 Thu, 09:00–16:00, EG L 12 |
| SS 2020 | Proteomics Practical Course |
Ludwig, C.
Medard, G.
Meng, C.
Wilhelm, M.
Wilhelm, S.
Responsible/Coordination: Küster, B. |
Thu, 09:00–17:00, EG L 12 Fri, 09:00–17:00, EG L 12 |
| WS 2019/20 | Proteomics Practical Course |
Ludwig, C.
Meng, C.
Wilhelm, M.
Wilhelm, S.
Responsible/Coordination: Küster, B. |
Mon, 09:00–16:00, EG L 12 |
| SS 2019 | Proteomics Practical Course |
Ludwig, C.
Medard, G.
Meng, C.
Wilhelm, M.
Wilhelm, S.
Responsible/Coordination: Küster, B. |
Mon, 09:00–16:00, EG L 12 |
| SS 2018 | Proteomics Practical Course | ||
| WS 2017/8 | Proteomics Practical Course | ||
| SS 2017 | Proteomics Practical Course | ||
| WS 2016/7 | Proteomics Practical Course | ||
| SS 2016 | Proteomics Practical Course | ||
| WS 2015/6 | Proteomics Practical Course | ||
| SS 2015 | Proteomics Practical Course | ||
| WS 2014/5 | Proteomics Practical Course |