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||This lecture discusses experimental techniques that can be used to characterize the structure and mechanics of biomaterials. First, macro- and microrheological techniques for determining the viscoelastic properties of biomaterials are reviewed. Then, imaging techniques such as optical microscopy (including contrast enhancement, fluorescence, super-resolution microscopy), electron microscopy, atomic-force microscopy as well as scattering techniques (such as x-ray and neutron scattering) as well as NMR are discussed. Finally, techniques for micro- and nanostructuring and their applications in microfluidics are reviewed.