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Techniques and Data Analysis in Biophysics

Module PH2251

This module handbook serves to describe contents, learning outcome, methods and examination type as well as linking to current dates for courses and module examination in the respective sections.

Module version of SS 2017

There are historic module descriptions of this module. A module description is valid until replaced by a newer one.

Whether the module’s courses are offered during a specific semester is listed in the section Courses, Learning and Teaching Methods and Literature below.

available module versions
WS 2021/2WS 2020/1WS 2019/20WS 2018/9WS 2017/8SS 2017

Basic Information

PH2251 is a semester module in German or English language at Master’s level which is offered in winter semester.

This module description is valid to SS 2017.

If not stated otherwise for export to a non-physics program the student workload is given in the following table.

Total workloadContact hoursCredits (ECTS)
150 h 45 h 5 CP

Responsible coordinator of the module PH2251 in the version of SS 2017 was Friedrich Simmel.

Content, Learning Outcome and Preconditions


  • The measurement of physical processes
    measurement uncertainties and error analysis, noise, data aquisition, thermal forces and diffusion
  • Atomic force microscopy (AFM)
    Basics of AFM-based force spectroscopy and Imaging, Instrumentation and data aquisition, data analysis
  • Dynamic light scattering and flow cytometry
    Basics light scattering and instrumentation, data analysis
  • Gel electrophoresis
    Basics and data analysis

Learning Outcome

After successful participation in this module, the student is able to:

  • to understand the basics of data aquisition, natural and technical fluctuations in measurements, and measurement uncertainties
  • to understand thermal forces and diffusion in biological systems
  • to understand the instrumentation and data analysis of AFM-based force spectroscopy and imaging
  • to understand the basic principles and the application of light scattering
  • to understand flow cytometry and its applictions
  • to understand the basics of gel electrophoresis


No preconditions in addition to the requirements for the Master’s program in Physics.

Courses, Learning and Teaching Methods and Literature

Learning and Teaching Methods

oral presentation, power point presentation, blackboard presentation, discusion


lecture notes, additional literature, moodle


will be announced in the lecture

Module Exam

Description of exams and course work

There will be an oral exam of 25 minutes duration. Therein the achievement of the competencies given in section learning outcome is tested exemplarily at least to the given cognition level using comprehension questions and sample calculations.

For example an assignment in the exam might be:

  • What is the difference between Nyquist and 1/f noise?
  • What are the imaging regimes in AFM? Explain them!
  • How is light scattering utilized in flow cell cytometry?
  • What is native electrophoresis?
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