Modern Applications of Fluorescence

Module PH2126

This module handbook serves to describe contents, learning outcome, methods and examination type as well as linking to current dates for courses and module examination in the respective sections.

Basic Information

PH2126 is a semester module in German or English language at Master’s level which is offered in summer semester.

This Module is included in the following catalogues within the study programs in physics.

  • General catalogue of special courses
  • Specific catalogue of special courses for Biophysics

If not stated otherwise for export to a non-physics program the student workload is given in the following table.

Total workloadContact hoursCredits (ECTS)
150 h 40 h 5 CP

Responsible coordinator of the module PH2126 is Thorsten Hugel.

Content, Learning Outcome and Preconditions


Molecular Spectra, Franck-Condon Principle


Fluorescence spectroscopy

Fluorescence resonance energy transfer (FRET)

Fluorescence correlation spectroscopy (FCS and FCCS)

Superresolution microscopy (STED, PALM)

Detection systems (e.g CCD, APD)


Learning Outcome

After successful participation in this module the student is able to:
1. Understand absorption and emission spectra.

2. To choose and apply the best modern fluorescence methods for a certain problem.


no info

Courses, Learning and Teaching Methods and Literature

Courses and Schedule

VO 2 Moderne Fluoreszenzmethoden Hugel, T. Montag, 15:00–17:00

Learning and Teaching Methods

Frontalunterricht mit Demonstrationsexperimenten


Tafelanschrieb bzw. Präsentation,
Demonstrationsexperimente (Erklärungen zum Download),
Beispielvideos (z.T. zum Download),
Vorlesungsmitschrift zum Download,


Hecht, Optik
Zinth, Optik
Lakowicz, Principles of Fluorescence Spectroscopy

Weitere Quellen: -> Physics3

Module Exam

Description of exams and course work

In an oral exam the learning outcome is tested using comprehension questions and sample problems.

In accordance with §12 (8) APSO the exam can be done as a written test. In this case the time duration is 60 minutes.

Exam Repetition

There is a possibility to take the exam at the end of the semester.

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A journey of discovery to understanding our world at the subatomic scale, from the nuclei inside atoms down to the most elementary building blocks of matter. Are you ready for the adventure?


Biological systems, from proteins to living cells and organisms, obey physical principles. Our research groups in biophysics shape one of Germany's largest scientific clusters in this area.