Molecular Biophysics: Spectroscopic Methods
PH2012 is a semester module in German language at Master’s level which is offered in winter semester.
If not stated otherwise for export to a non-physics program the student workload is given in the following table.
|Total workload||Contact hours||Credits (ECTS)|
|150 h||60 h||5 CP|
Responsible coordinator of the module PH2012 is Klaus Achterhold.
Content, Learning Outcome and Preconditions
- optical absorption
- reaction kinetics
- enzyme kinetics
- normal modes
- Fourier transform infrared spectroscopy (FTIR)
- Raman spectroscopy
- circular dichroism and optical rotatory dispersion
- EPR spectroscopy (Electron Paramagnetic Resonance)
- NMR spectroscopy (Nuclear Magnetic Resonance)
- NMR tomography
- electron nuclear double resonance spectroscopy (ENDOR)
- extended X-ray absorption fine structure ( EXAFS)
- Mössbauer spectroscopy with synchrotron radiation and radioactive sources
After successfully participating in this module controls the students understand the basics of a variety of spectroscopic methods. The students know how the amount of secondary structure elements of a protein, local distances of atoms in proteins and the chemical environment of the probe atoms in the protein can be determined. Furthermore, the students have learned, how the harmonic and the protein-specific dynamics are determined, and which information about the function of a protein is obtained.
No preconditions in addition to the requirements for the Master’s program in Physics.
Courses, Learning and Teaching Methods and Literature
Courses and Schedule
|VU||4||Spectroscopic methods in molecular biophysics||Achterhold, K.||
Mon, 16:00–17:30, PH 2074
and dates in groups
Learning and Teaching Methods
Lecture, beamer presentation, discussion
- Tilman Butz, Fouriertransformation für Fußgänger, Teubner
- Hans-Joachim Galla, Spektrosk. Methoden in der Biochemie, Thieme Verlag
- Horst Friebolin, Ein- und zweidimensionale NMR-Spektroskopie, VCH
- John E. Wertz, James R. Bolton, Electron Spin Resonance, Elementary Theory and Practical Applications, McGraw-Hill
- Topics in Applied Physics, Vol 5, Mössbauer Spectroscopy, Ed. U. Gonser, Springer Verlag
Description of exams and course work
In an oral exam the learning outcome is tested using comprehension questions and sample problems.
In accordance with §12 (8) APSO the exam can be done as a written test. In this case the time duration is 60 minutes.
The exam may be repeated at the end of the semester. There is a possibility to take the exam in the following semester.