Mammalian Cell Engineering
This module handbook serves to describe contents, learning outcome, methods and examination type as well as linking to current dates for courses and module examination in the respective sections.
CH0170 is a semester module in English language at Master’s level which is offered every semester.
This Module is included in the following catalogues within the study programs in physics.
- Focus Area Bio-Sensors in M.Sc. Biomedical Engineering and Medical Physics
|Total workload||Contact hours||Credits (ECTS)|
|180 h||60 h||6 CP|
Content, Learning Outcome and Preconditions
The lectures and exercises will transport the following content:
1. Central dogma of molecular biology: DNA to RNA to Protein
2. Layers of regulation and their amenability to engineering
3. Genetic circuits in mammalian cells
4. Technical excursion: State-of-the-art molecular cloning techniques
5. Classes of gene reporters
6. Principles of protein engineering: de novo designs and rational designs based on circular permutations of proteins, split-protein technology, modeling-based protein engineering, directed evolution, library designs, in vitro diversification, error-prone PCRs.
7. Genetically encoded sensors of analytes or cellular states (GECIs, GEVIs other neurotransmitter indicators, protease amplifiers, split sensors)
8. Gene editing: From Zinc fingers, via TALENs to CRISPR/Cas based technology
9. Genetically controlled actuators (chemogenetics, optogenetics, and variants)
10. Towards applications in cellular theranostics and regenerative medicine
● understand the basic processes in mammalian cells and how they can be accessed by biological engineering;
● understand the options for the analysis and manipulation of cellular processes via genetically encoded components;
● understand gene editing methodology;
● have gained insight into current and future applications for cellular theranostics and regenerative medicine.
Courses, Learning and Teaching Methods and Literature
Courses and Schedule
|VI||4||Mammalian Cell Engineering (CH0170)||Westmeyer, G.||
Learning and Teaching Methods
● B. Alberts, A. Johnson, D. Morgan, M. Raff, K. Roberts & P. Walter: Molecular Biology of the Cell, Norton & Company, (2014)
● Ron Milo, Rob Phillips: Cell Biology by the numbers; http://book.bionumbers.org/